Chemical synthesis of RNA with site-specific methylphosphonate modifications

Chemical synthesis of RNA with site-specific methylphosphonate modifications

Methylphosphonate(mP)-modified RNA serves as valuable probe to evaluate biomolecular interactions between the nucleic acid backbone and binding partners, such as proteins or small molecules. Here, we describe an efficient workflow for the synthesis of RNA with a single mP modification in diastereomerically pure form. While the automated assembly of mP-modified RNA is straightforward, its deprot...

Site-specific chemical labeling of long RNA molecules.

Site-specific labeling of RNA molecules is a valuable tool for studying their structure and function. Here, we describe a new site-specific RNA labeling method, which utilizes a DNA-templated chemical reaction to attach a label at a specific internal nucleotide in an RNA molecule. The method is nonenzymatic and based on the formation of a four-way junction, where a donor strand is chemically co...

Site-specific chemical modification procedures.

Synthesis of phosphorothioate-methylphosphonate oligonucleotide co-polymers.

We describe the synthesis and characterization of novel phosphorothioate-methylphosphonate backbone-modified oligodeoxynucleotide co-polymers. These may have potential use as antisense constructs.

Chemical Modifications and Kinetic Study of Ribonuclease Sa Active Site

Chemical modification experiments and kinetic measurements have been carried out to identify the active site components of guanylspecific ribonuclease Sa (E. C. 3.1.4.8.) from Streptomyces aureofaciens. Modification experiments with phenylglyoxal and diketene showed that neither arginine nor lysine residues, which could bind the negatively charged phosphate group of the substrate, belonged to t...